abstract：:Rapid, nongenomic in vitro effects of aldosterone on intracellular electrolytes, cell volume and the sodium-proton antiporter have been found in human mononuclear leukocytes (HML), as have related membrane receptors. In the present study, binding of 125I-labeled aldosterone to plasma membrane preparations from pig kid...

journal_title：Molecular and cellular endocrinology

authors： Christ M,Sippel K,Eisen C,Wehling M

更新日期：1994-03-01 00:00:00

abstract：:Molecular cloning of the prolactin (PRL) receptor cDNA has revealed different forms of the receptor: among them, the longest form encodes a transmembrane protein of 592-598 amino acids and was originally found in rabbit mammary gland as well as in human and rat tissues. It contains a cytoplasmic domain of 358 amino ac...

journal_title：Molecular and cellular endocrinology

authors： Genty N,Paly J,Edery M,Kelly PA,Djiane J,Salesse R

更新日期：1994-03-01 00:00:00

abstract：:Corticotropin (ACTH) binds to specific receptors in the adrenal cortex and thereby regulates glucocorticoid and mineralocorticoid production. The number of ACTH binding sites on adrenocortical cells is increased by exposure of cells to activators of the cAMP pathway. The mechanism responsible for the increase in ACTH ...

journal_title：Molecular and cellular endocrinology

authors： Mountjoy KG,Bird IM,Rainey WE,Cone RD

更新日期：1994-02-01 00:00:00

abstract：:Transgenic mice carrying a fused gene of the 294-base upstream and 68-base leader sequences of a rat calmodulin gene, CaMII, and beta-galactosidase gene were made. Only spermatocytes expressed the transgene mRNA in the testes of four independent transgenic lines. The localization of transgene mRNA was consistent with ...

journal_title：Molecular and cellular endocrinology

authors： Ikeshima H,Shimoda K,Matsuo K,Hata J,Maejima K,Takano T

更新日期：1994-02-01 00:00:00

abstract：:Calcium is a second messenger responsible for regulating a wide range of cellular processes. It is normally presented as brief spikes even in non-excitable cells. The necessity of limiting the period of calcium stimulation to brief bursts may depend upon the fact that prolonged elevation of calcium can be toxic. It ca...

journal_title：Molecular and cellular endocrinology

authors： Berridge MJ

更新日期：1994-01-01 00:00:00

abstract：:Tyrosine kinase activation in mediating the mitogenic action of prolactin (PRL) has been evaluated. Use was made of genistein, a tyrosine kinase antagonist, and cultured rat Nb2 lymphoma cells, i.e. the lactogen-dependent Nb2-11 line and a lactogen-independent subline, Nb2-SFJCD1. Genistein was found to be a potent gr...

journal_title：Molecular and cellular endocrinology

authors： Buckley AR,Buckley DJ,Gout PW,Liang H,Rao YP,Blake MJ

更新日期：1993-12-01 00:00:00

abstract：:The apparent preferential expression of the elastase/cathepsin G protease inhibitor antileukoproteinase (ALP) in endometrium of species with epitheliochorial placenta suggests mechanisms of transcriptional regulation unique to these mammalian species. To begin to define the cis-acting regulatory elements involved in t...

journal_title：Molecular and cellular endocrinology

authors： Simmen RC,Badinga L,Michel FJ

更新日期：1993-11-01 00:00:00

abstract：:Estrogens are synthesized from C19 steroids by a unique form of cytochrome P450, aromatase cytochrome P-450 (P-450AROM; the product of the CYP19 gene). We have shown that tissue-specific expression of human P-450AROM is determined, in part, by the use of alternative promoters. Previous methods of analysis for determin...

journal_title：Molecular and cellular endocrinology

authors： Jenkins C,Michael D,Mahendroo M,Simpson E

更新日期：1993-11-01 00:00:00

abstract：:The most potent, physiologic activator of proopiomelanocortin (POMC) gene transcription is corticotropin releasing hormone (CRH) and increased intracellular cAMP is critical for this effect. The 5'-flanking region of the murine POMC gene has several potential binding sites for regulatory proteins. To characterize the ...

journal_title：Molecular and cellular endocrinology

authors： Bishop JF,Mouradian MM

更新日期：1993-11-01 00:00:00

abstract：:The cell line MD-IGF-1, containing an ovine IGF-1 cDNA driven by the mouse mammary tumor virus-long terminal repeat (MMTV-LTR) promoter, was used to study expression of IGF-1 linked to the MMTV-LTR in bovine mammary epithelial cells in response to various hormonal and substratum stimuli. Acute sensitivity of the MMTV-...

journal_title：Molecular and cellular endocrinology

authors： Romagnolo D,Akers RM,Wong EA,Boyle PL,McFadden TB,Byatt JC,Turner JD

更新日期：1993-10-01 00:00:00

abstract：:The testicular Sertoli cell produces an extracellular androgen-binding protein (ABP) that binds testosterone and dihydrotestosterone with high affinity. The ABP gene also encodes plasma sex hormone-binding globulin (SHBG), which is produced by the liver of most species. Unlike the human, adult rats and mice do not exp...

journal_title：Molecular and cellular endocrinology

authors： Reventos J,Sullivan PM,Joseph DR,Gordon JW

更新日期：1993-10-01 00:00:00

abstract：:A pair of glands (Y-organs) in crustaceans synthesize and secrete ecdysteroid hormones; the obligate precursor for synthesis is circulating cholesterol. Ecdysteroid output by the Y-organs is regulated negatively by an eyestalk neurosecretory peptide, molt-inhibiting hormone (MIH). The question was addressed, does MIH ...

journal_title：Molecular and cellular endocrinology

authors： Spaziani E,Wang WL

更新日期：1993-09-01 00:00:00

abstract：:The protein kinase C (PKC) activator, phorbol 12,13-dibutyrate (PDBu) induced the release of both luteinizing hormone (LH) and growth hormone (GH) from proestrous rat anterior pituitary pieces in vitro. Phorbol 12,13-dibutyrate-induced LH, but not GH release was readily inhibited by the phospholipase A2 (PLA2) inhibit...

journal_title：Molecular and cellular endocrinology

authors： Thomson FJ,Mitchell R

更新日期：1993-09-01 00:00:00

abstract：:The expression of the three genes encoding the components C1, C2 and C3 of prostatic binding protein (PBP) is under androgen control and restricted to the rat ventral prostate. The SstI-PvuII fragment of the first intron of the C3(1) gene displays two binding sites for ubiquitous transcription factors and one for a ti...

journal_title：Molecular and cellular endocrinology

authors： Celis L,Claessens F,Peeters B,Heyns W,Verhoeven G,Rombauts W

更新日期：1993-08-01 00:00:00

abstract：:The expression of genes encoding inhibin/activin subunits and activin receptor was examined in four cultured Leydig tumor cells (MA-10, I-10, R2C, and LC-540). Inhibin alpha-subunit gene was highly expressed in Leydig tumor cell lines except LC-540. Both inhibin beta-A- and beta-B-subunit mRNAs were present in low lev...

journal_title：Molecular and cellular endocrinology

authors： Chen CL,Pignataro OP,Feng ZM

更新日期：1993-07-01 00:00:00

abstract：:Transcriptional regulation of the human parathyroid hormone-related protein (PTHrP) gene by calcitonin was examined in a lung cancer line (BEN cells). Northern analysis demonstrated that calcitonin caused a rapid 4.5-fold elevation in PTHrP mRNA. Transient transfection of a construct containing 1119 base pairs of the ...

journal_title：Molecular and cellular endocrinology

authors： Chilco PJ,Gerardi JM,Kaczmarczyk SJ,Chu S,Leopold V,Zajac JD

更新日期：1993-07-01 00:00:00

abstract：:Rat thyroid tissue and cultured rat thyrocyte lines contain two thyroglobulin (Tg) mRNAs: a 9 kb rTg-1 mRNA encoding the 330, kDa Tg monomer and a recently described 0.95 kb rTg-2 mRNA. These transcripts have identical 5' coding sequences (641 nucleotides); however, the 3' end of rTg-2 is comprised of coding and non-c...

journal_title：Molecular and cellular endocrinology

authors： Graves PN,Davies TF

更新日期：1993-06-01 00:00:00

abstract：:Receptor-mediated Ca2+ entry was investigated in fura-2-loaded thyroid FRTL-5 cells. Activation of protein kinase C (PKC) by phorbol myristate acetate (PMA) attenuated the ATP-induced increase in intracellular free Ca2+ ([Ca2+]i). In PKC down-regulated cells, the ATP-induced increase in [Ca2+]i was increased compared ...

journal_title：Molecular and cellular endocrinology

authors： Törnquist K

更新日期：1993-05-01 00:00:00

abstract：:Pituitary cultures from adult rats contain two subtypes of prolactin (PRL) cells, small-plaque (SP) and large-plaque (LP) lactotropes, which exhibit distinct rates of basal secretion and thereby form PRL plaques of different sizes in reverse hemolytic plaque assay experiments. In the present study, we have used plaque...

journal_title：Molecular and cellular endocrinology

authors： Horta J,Cota G

更新日期：1993-04-01 00:00:00

abstract：:Five overlapping synthetic peptides representing two regions of thyrotropin (TSH) binding sites of human thyrotropin receptor (TSHR) (peptides 12-30, 24-44, 308-328, 324-344 and 339-364) were investigated for their ability to cause proliferation of peripheral blood lymphocytes (PBL) from eight patients with Graves' di...

journal_title：Molecular and cellular endocrinology

authors： Sakata S,Tanaka S,Okuda K,Miura K,Manshouri T,Atassi MZ

更新日期：1993-03-01 00:00:00

abstract：:Studies were conducted in vitro and in vivo to determine whether or not inhibin affects the transcription rate of the gene for the beta subunit of follicle stimulating hormone (FSH beta). Pituitary cells in primary culture were incubated with 0-3000 milli-units/ml inhibin; a dose-related decrement in mRNA was obtained...

journal_title：Molecular and cellular endocrinology

authors： Clarke IJ,Rao A,Fallest PC,Shupnik MA

更新日期：1993-02-01 00:00:00

abstract：:Incubation of pituitary GH1 cells with N'-methylnicotinamide, nicotinamide and 3-acetylpyridine which inhibit nuclear ADP-ribosylation and/or the cellular concentration of its substrate NAD+ reduced the amount of nuclear thyroid hormone receptors in a time- and dose-dependent manner without altering the affinity of th...

journal_title：Molecular and cellular endocrinology

authors： Sanchez-Pacheco A,Perez P,Villa A,Pascual A,Aranda A

更新日期：1993-02-01 00:00:00

abstract：:Relaxin is a peptide hormone which consists of two polypeptide chains that are synthesized as a B-chain/C-peptide/A-chain precursor. We have used the polymerase chain reaction (PCR) to isolate and clone a relaxin-like cDNA from sheep placental RNA. This cDNA and two sheep genomic clones were characterised by nucleotid...

journal_title：Molecular and cellular endocrinology

authors： Roche PJ,Crawford RJ,Tregear GW

更新日期：1993-02-01 00:00:00

abstract：:We previously reported that kappa opioids stimulated the release of human placental lactogen (hPL) from trophoblastic cells and that this effect was prevented by co-incubation with naloxone. We also reported that adenylate cyclase was not directly involved in this process. In order to understand the post-receptor even...

journal_title：Molecular and cellular endocrinology

authors： Petit A,Gallo-Payet N,Bellabarba D,Lehoux JG,Bélisle S

更新日期：1993-01-01 00:00:00

abstract：:In Manduca sexta, basal and PTTH-stimulated secretion of ecdysteroids by prothoracic glands in vitro increases from days 1 to 4 of the fifth larval stage. Glandular content of cAMP-dependent protein kinase was analyzed to determine if the enzyme changes in concert with increased secretory response. Photoaffinity label...

journal_title：Molecular and cellular endocrinology

authors： Smith WA,Varghese AH,Lou KJ

更新日期：1993-01-01 00:00:00

abstract：:Fibroblasts from three patients with vitamin D-dependency rickets type II were used to study mutations in the 1,25-dihydroxyvitamin D3 receptor responsible for this hereditary disease. Normal human fibroblasts contain 43 +/- 13 fmol receptor/mg protein as determined by immunoradiometric assay and 22 +/- 3 fmol/mg by l...

journal_title：Molecular and cellular endocrinology

authors： Wiese RJ,Goto H,Prahl JM,Marx SJ,Thomas M,al-Aqeel A,DeLuca HF

更新日期：1993-01-01 00:00:00

abstract：:To define the heparin-binding site of follistatin, the reduced and S-carboxymethylated recombinant human follistatin containing 288 amino acids was digested by Staphylococcus aureus V8. The digested product was subjected to sulfate cellufine column chromatography and the adsorbed peptide fragments eluted with a stepwi...

journal_title：Molecular and cellular endocrinology

authors： Inouye S,Ling N,Shimasaki S

更新日期：1992-12-01 00:00:00

abstract：:The antiestrogen tamoxifen has been successfully used to control estrogen receptor (ER) and progesterone receptor positive breast cancer. However, the development of antiestrogen resistance is frequently observed in patients following long term treatment. We have studied the development of antiestrogen resistance in v...

journal_title：Molecular and cellular endocrinology

authors： Jiang SY,Wolf DM,Yingling JM,Chang C,Jordan VC

更新日期：1992-12-01 00:00:00

abstract：:The role of AII receptors subtypes, AT1 and AT2, in the regulation of aldosterone secretion was studied in adrenal glomerulosa cells and membranes from rats on normal and low sodium intake, using AII receptor subtype-specific antagonists. In adrenal glomerulosa cells, more than 90% of the receptors were AT1 and there ...

journal_title：Molecular and cellular endocrinology

authors： Aguilera G

更新日期：1992-12-01 00:00:00

abstract：:Stanniocalcin (STC) (formerly known as both teleocalcin and hypocalcin) is an anti-hypercalcemic, glycoprotein hormone that is produced by the corpuscles of Stannius (CS), endocrine glands that are confined to bony fishes. The hormone has a unique amino acid sequence and exists as a disulfide-linked homodimer in the n...

journal_title：Molecular and cellular endocrinology

authors： Wagner GF,Dimattia GE,Davie JR,Copp DH,Friesen HG

更新日期：1992-12-01 00:00:00

abstract：:Glucose uptake by brown adipose tissue, measured following deoxyglucose injection in vivo, was increased by 6- and 11-fold following 2 and 14 days of cold exposure, respectively. To look for the possible mechanism of these modifications, the glucose transporter Glut 4 has been characterized at the protein and mRNA lev...

journal_title：Molecular and cellular endocrinology

authors： Olichon-Berthe C,Van Obberghen E,Le Marchand-Brustel Y

更新日期：1992-11-01 00:00:00

abstract：:In an effort to identify the signal transduction mechanism associated with the inhibition of juvenile hormone (JH) biosynthesis by the neuropeptides allatostatins, levels of the cyclic nucleotides cAMP and cGMP were measured in corpora allata (CA) of virgin and mated Diploptera punctata females using radioimmunoassays...

journal_title：Molecular and cellular endocrinology

authors： Cusson M,Yagi KJ,Guan XC,Tobe SS

更新日期：1992-11-01 00:00:00

abstract：:Clusterin, alpha 2-macroglobulin and testins are three novel Sertoli cell proteins whose physiological functions may be related to cell-cell interactions in the seminiferous epithelium of the testis. We have demonstrated the biosynthesis of clusterin, alpha 2-macroglobulin, and testins by Sertoli cells in vitro using ...

journal_title：Molecular and cellular endocrinology

authors： Grima J,Pineau C,Bardin CW,Cheng CY

更新日期：1992-11-01 00:00:00

abstract：:The major ganglioside NeuAc alpha 2-->3Gal beta 1-->4Glc beta 1-->1Cer (GM3) present in cultured rat granulosa cells was examined for potential function in the expression of luteinizing hormone (LH) receptor on the cell surface in response to follicle-stimulating hormone (FSH). Synthesis of GM3 was stimulated concentr...

journal_title：Molecular and cellular endocrinology

authors： Hattori M,Horiuchi R

更新日期：1992-10-01 00:00:00

abstract：:As part of an ongoing study of the cell-specific expression of glandular kallikrein genes in mice, we have investigated cellular sites of expression of the renal/pancreatic kallikrein gene, mGK-6, during fetal life. Expression of alpha I and beta-subunit genes of Na+K+ATPase and bradykinin binding were used as an indi...

journal_title：Molecular and cellular endocrinology

authors： Penschow JD,Coghlan JP

更新日期：1992-10-01 00:00:00

abstract：:Recent evidence suggests that lymphocytes produce prolactin (PRL). Here, we report the cDNA cloning and expression of PRL from normal human thymocytes. Sequence analysis showed that the thymocyte cDNA encodes a 23 kDa protein which is identical to pituitary PRL. RNA blot analysis showed that the thymocyte PRL mRNA is ...

journal_title：Molecular and cellular endocrinology

authors： O'Neal KD,Montgomery DW,Truong TM,Yu-Lee LY

更新日期：1992-09-01 00:00:00

abstract：:Glandular epithelial (GE) and stromal cells were isolated from guinea-pig endometrium, cultured and subcultured separately. At the end of subculture, the purity of each cell population was higher than 95% and cells displayed a high level of estrogen receptors. Calcium phosphate transfection conditions were defined usi...

journal_title：Molecular and cellular endocrinology

authors： Vuillermoz C,Jouvenot M,Pellerin I,Ordener C,Royez M,Adessi GL

更新日期：1992-09-01 00:00:00

abstract：:As deduced on the basis of cloning experiments, the putative extracellular domain of pituitary glycoprotein hormone (lutropin (LH), thyrotropin (TSH), and FSH) receptors (rec) is sufficiently large to suggest involvement in hormone binding. Comparison of the amino acid sequences of the extracellular domains of the gly...

journal_title：Molecular and cellular endocrinology

authors： Dattatreyamurty B,Reichert LE Jr

更新日期：1992-09-01 00:00:00

abstract：:Carboxypeptidase H (CP-H) has been characterized in anterior and posterior lobes of bovine pituitary with regard to subcellular distribution, enzymatic properties, nature of membrane association, number of forms and C-terminal processing. Anterior lobe contained both soluble and membrane forms of CP-H with similar enz...

journal_title：Molecular and cellular endocrinology

authors： Parkinson D

更新日期：1992-08-01 00:00:00

abstract：:Using dispersed cultures of fetal rat hypothalami, we studied the effects of forskolin and the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA), activators of protein kinase A and C, respectively, upon vasopressin (VP) secretion, VP mRNA expression and VP mRNA poly(A) tail length. Forskolin stimulated the VP m...

journal_title：Molecular and cellular endocrinology

authors： Emanuel RL,Girard DM,Thull DL,Majzoub JA

更新日期：1992-07-01 00:00:00